Fig. 3: M. tuberculosis manipulate IL-1β induction independent of host TB status. | Nature Communications

Fig. 3: M. tuberculosis manipulate IL-1β induction independent of host TB status.

From: Mycobacterium tuberculosis associated with severe tuberculosis evades cytosolic surveillance systems and modulates IL-1β production

Fig. 3

M. tuberculosis isolates 4I2 and 6C4 were selected based on the TB presentation, MTBC sublineage and intensity of cytokine induction in infected PBMCs. PBMCs from IGRA +  (a) or IGRA– (b) donors; CD14 + monocytes purified from IGRA– donors (c); PMA-differentiated THP-1 cells (d) or C57BL/6 mouse BMDMs (e) were infected with M. tuberculosis isolate 4I2 (solid circles or bars) or 6C4 (open squares or bars) for 24 h and the amount of secreted IL-1β quantified in the culture supernatants by immunoassay. MOIs of 1 (a–d) or 2 (e) were used for infection. Represented is the mean ± SEM; n = 10 donors (a, b), or n = 3 donors (c) or n = 3 wells from one (d) or four (e) independent experiments. Undetected values are not represented. Statistical analysis was performed using two-tailed unpaired Student’s t-test (*p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001).

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