Fig. 3: Fed-batch fermentation profiles of various metabolically engineered M. succiniciproducens strains.
From: Enhanced succinic acid production by Mannheimia employing optimal malate dehydrogenase
Fed-batch fermentations of the a PALKcgmdh and b PALKmsmdhG11Q strains were carried out in CDM using glucose and glycerol as dual carbon sources. Fed-batch fermentations of the c PALK and d PALKcgmdh strains were carried out using glucose and glycerol with increased initial cell mass (OD600 = 21.1 and 19.3, respectively). Symbols: White square, cell growth; red circle, SA; black square, glucose; black diamond, glycerol; white circle, pyruvate; white diamond, acetate; white triangle, formate. Fermentations were all performed in duplicate (n = 2 independent experiments). The fermentation profile shown here represents the result of one fed-batch culture, whereas the result of another reproduced fed-batch culture is shown in Supplementary Fig. 15. Source data are provided as a Source Data file.
