Fig. 1: EDC-induced metabolic reprogramming in the setting of a Western-style diet. | Nature Communications

Fig. 1: EDC-induced metabolic reprogramming in the setting of a Western-style diet.

From: Epigenome environment interactions accelerate epigenomic aging and unlock metabolically restricted epigenetic reprogramming in adulthood

Fig. 1

a Schematic of the integrated longitudinal epigenomic, transcriptomic and metabolomic analyses performed across the life-course in a rat model of early-life (postnatal days (PND)1–5) exposure to the EDC bisphenol A. Techniques and analytic approaches utilized at each life-stage are shown. b Metabolic phenotyping of vehicle- and EDC-exposed animals fed a Western-style diet at D240. Black circles represent individual vehicle (VEH) and red squares represent individual EDC (EDC) animals. N = 7 biologically independent animals per treatment. The p values generated by t test are indicated. *p < 0.05; **p < 0.01; ***p < 0.001. c EDC-exposure causes dyslipidemia in exposed animals fed a Western-style diet as seen in the targeted serum lipidomic analysis of vehicle- and EDC-exposed animals (N = 5 biologically independent animals per treatment). This analysis identified phospholipid [phosphatidylglycerol (PG), phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidic acid (PA), phosphatidylcholine (PC), and phosphatidylinositol (PI)], cardiolipin (CL), cholesteryl ester (CE), diacylglycerol (DG), triglyceride (TG), and plasmenyl-PE levels as significantly (q < 0.25) increased in EDC-exposed animals on Western-style diet compared to VEH-exposed animals fed the same diet. Each bar is a different specific lipid within a color-coded class for each lipid. Source data for b, c are provided as a Source data file.

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