Table 1 Selection of targets and MiSeq verification of knockouts.
From: Multiplex secretome engineering enhances recombinant protein production and purity
Cell line | Target | Protein name | Uniprot | Rationale | Location | Frameshift |
|---|---|---|---|---|---|---|
6xKO | BGN | Biglycan | G3HSX8 | A | Secreted | +1 |
TIMP1 | Metalloproteinase inhibitor 1 | G3IBH0 | A/C | Secreted | −5/−2/+1 | |
LGALS3BP | Galectin-3-binding protein | G3H3E4 | A/C | Membrane | +33 | |
NID1.1 | Nidogen-1 | G3HWE4 | A/C | Membrane/matrix | +1 | |
NID1.2 | Nidogen-1 | G3I3U5 | A/C | Membrane/matrix | +1 | |
CTSD | Cathepsin D | G3I4W7 | A/C/Q | Lysosome | −8 | |
11xKO | Aga | N(4)-(Beta-N-acetylglucosaminyl)-l-asparaginase | G3HGM6 | A | Secreted | −40 |
Erp29 | Endoplasmic reticulum resident protein 29 | G3H284 | A | ER | −1 | |
Gpr56 | G-protein coupled receptor 56 | G3I3K5 | A/C | Membrane | +1 | |
Tinagl1 | Tubulointerstitial nephritis antigen-like | G3H1W4 | A/C | Secreted | +1 | |
Lgmn | Legumain | G3I1H5 | A/C/Q | Lysosome | +1 | |
14xKO | Yeats2 | YEATS domain-containing protein 2 | G3I3I8 | A | Nucleus | +1 |
Sparc | SPARC | G3H584 | A/C | Secreted | −5 | |
Lpl | Lipoprotein lipase | G3H6V7 | A/C/Q | Membrane/secreted | +1 |
