Fig. 8: The global structure of NME1 but not TLC1 is affected in pop cells at 24 °C.

DMS-MaPseq analysis from strains grown at 24 °C for ~50 generations. a Four regions of NME1 RNA with statistically significant different DMS reactivities in pop1 (blue bars) and pop6 (purple bars) compared to WT (gray bars) cells are shown. DMS reactivities from three independent biological replicates were averaged. b q Values and Δd values of regions 1–4 of NME1 RNA in pop1 and pop6 compared to WT cells. Regions of five nucleotides (or more) that had q-values < 0.1 and Δd > 0.01 were considered statistically significant. c The four regions shown in (A) are mapped onto the NME1 RNA structure²³; region numbers are the same as in a. The significantly altered regions are denoted by red boxes. d Average normalized DMS reactivity from nucleotides 608–615 in TLC1. These nucleotides are at the site of Pop6/7 binding. Symbols are the same as in panel A. q values and Δd values of the indicated region in pop1 and pop6 compared to WT. e Enlarged view of the CS2a/TeSS domain of TLC1 indicated in d. Nucleotides in orange indicate the only nts in TLC1 that met the criteria for being differentially reactive. See supplementary Figs. 4–7 for DMS reactivity in regions where there was no significant difference between pop mutants and WT. Source data are provided as a Source Data file.