Fig. 2: Targeted resequencing of genes in the S/MAPK pathways.

a Schematic view of the strategy used to obtain nitrogen-starved cultures. The same progenitor strain was used to grow (+N) three independent cultures (in addition to culture 0) prior to shifting to quiescence (−N) (left panel). The same approach was used to grow a single culture prior to splitting on day 1 of quiescence into six parallel subcultures (right panel). Samples were analyzed after 2 and 3 months of starvation. b Distribution and percentage of mutations found in the thousand colonies picked from cultures 0 to 3 after 2 months. The alleles in red were confirmed by Sanger sequencing the stress-sensitive colonies found among 120 cells plated out from the frozen pools. c same as b after 3 months. d Distribution and percentage of mutations in the subcultures after 2 months of starvation.