Fig. 5: Mucosal induction of adaptive immunity by live STm^Aux is robust in MYD88/TRIF double-deficient mice.

a Germ-free MYD88^−/−TRIF^lps/lps mice (open symbols) and wild-type control mice (filled symbols) were enterally conditioned with three doses of 10¹⁰ CFU of live STm^Aux (red triangles, n = 7 MYD88/TRIF KO animals and n = 9 wild-type animals examined over two independent experiments) or left untreated as controls (gray circles, n = 6 MYD88/TRIF KO animals and n = 10 wild-type animals examined over two independent experiments). Twenty-seven days after the first treatment (day 0) mice were challenged with wild-type STm (10³ CFU) harboring ssag::eGFP reporter plasmid pM973. The mice were studied at day 3 after challenge. Each symbol represents one individual. b Quantification of intracellular wild-type STm expressing eGFP in the cecal mucosa. c Bacterial burden of wild-type STm recoverable from mLN, spleen, and liver at day 3 after challenge. Statistics: bars indicate mean (b, c). Horizontal, dotted lines indicate the lower detection limit (b, c). Panels b and c were analyzed with two-way ANOVA (host genotype and treatment as factors) and Sidak multiple comparison correction. The data were pooled from two independent experiments. Source data are provided as a Source Data file. Detailed statistical metrics are available in the Supplementary Statistical Analysis file.