Table 1 Bacterial strains and plasmids.
From: Uncoupling of invasive bacterial mucosal immunogenicity from pathogenicity
Strain (acronym)/plasmid | Genetic background | Relevant genotype | Known resistances | Comments | Origin or reference |
|---|---|---|---|---|---|
χ4138 (STm) | UK-1 | Wild-type strain | Nal | Virulent wild-type control | |
χ9650 (STmΔSPI1 ΔSPI2) | UK-1 | Δ(avrA-invH::cat) Δ(ssaG-ssaU::aphT | Nal, Cam, Kan | ||
χ9052 | UK-1 | Δalr3 ΔdadB4 ΔasdA33 | |||
M736 | ATCC 14028s derivate IR715 (ref. 69) | ΔinvC::aphT | Nal, Kan | ||
M738 | ATCC 14028s derivate IR715 (ref. 69) | ΔssaV::cat | Nal, Cam | ||
HA135 (STmAux) | UK-1, χ9052 | ΔmetC::tetRA Δalr3 ΔdadB4 ΔasdA33 | Tet | This study | |
HA618 (STmAux ΔinvC) | UK-1, STmAux | ΔmetC::tetRA Δalr3 ΔdadB4 ΔasdA33 ΔinvC::aphT | Tet, Kan | This study | |
HA218 (STmAux ΔinvC ΔssaV) | UK-1, STmAux | ΔmetC::tetRA Δalr3 ΔdadB4 ΔasdA33 ΔinvC::aphT ΔssaV::cat | Tet, Kan, Cam | This study | |
HA208 (STmAux ΔSPI1 ΔSPI2) | UK-1 | ΔmetC::tetRA Δalr3 ΔdadB4 ΔasdA33 Δ(avrA-invH::cat) Δ(ssaG-ssaU::aphT | Tet, Kan, Cam | This study | |
pM973 | pWKS30 (ref. 70) | Amp | eGFP under control of the ssaG promoter | ||
SB300 (STm) | SL1344 | Wild-type strain | Str | ||
HA623 | SL1344 | ΔmetC ΔalrN ΔalrP Δasd | Str | This study | |
HA630 (STmAux) | SL1344, HA300 | ΔmetC ΔalrN ΔalrP Δasd::tetRA | Str, Tet | Used in Fig. 7 and corresponding Supplementary figures | This study |
HA705 (STmT3SS−) | SL1344, SB300 | ΔinvC::aphT ΔssaV::cat | Str, Kan, Cam | Used in Fig. 7 | This study |
HA706 (STmΔssaV) | SL1344, SB300 | ΔssaV::cat | Str, Cam | Used in Fig. 7 | This study |
HA702 (STmAux T3SS−) | SL1344, SB300 | ΔmetC ΔalrN ΔalrP Δasd::tetRA ΔinvC::aphT ΔssaV::cat | Str, Tet, Kan, Cam | Used in Fig. 7 | This study |
HA700 (STmAux ΔinvC) | SL1344, SB300 | ΔmetC ΔalrN ΔalrP Δasd::tetRA ΔinvC::aphT ΔinvC::aphT | Str, Tet, Kan | Used in Fig. 7 | This study |
HA727 (STmAux ΔTriple-Eff) | SL1344, SB300 | ΔmetC ΔalrN ΔalrP Δasd ΔinvC::aphT ΔinvC::aphT ΔsopE::pGP704 ΔsipA::aphT ΔsopE2::pM218 | Str, Tet, Kan, Cam, | Used in Fig. S7 | This study |
HA733 (STmΔTriple-Eff) | SL1344, SB300 | ΔmetC ΔalrP ΔsopE::pGP70 ΔsipA::aphT ΔsopE2::pM218 | Str, Tet, Kan, Cam | Prototrophic derivative of HA727 | This study |
SKI12 (rough STm) | SL1344, SB300 | ΔwbaP | Nal | ||
M1525 (SEn) | S. Enteritidis 125109 | Wild-type strain | |||
HA627 (rough SEn) | S. Enteritidis 125109 | ΔrfbS | This study |
