Table 2 Primers used for bacterial mutagenesis.
From: Uncoupling of invasive bacterial mucosal immunogenicity from pathogenicity
Primer name | 5′ → 3′ sequence | Additional information | Applicable background |
|---|---|---|---|
STM-metC-tetRA-F | TTTGGCAAAATTTTCATCTGTATCACACGTCGCCAGGGTGCAGATGGTTATATTCATGCTAGTTTAGACATCCAGACGGTTAAAATCAGGAAACGCAACTTAAGACCCACTTTCACATT | Allelic exchange of metC with TetRA | UK-1 |
STM-metC-tatRA-R | GTACTCCTGAATCGTCCGGGATGCCTTGATCCCGGACGCAACAAACGCAGACTTTTCCACGGAAATTGTCTGCATATATGTCCATCCCCGGCAACTTTACTAAGCACTTGTCTCCTG | Allelic exchange of metC with TetRA | UK-1 |
STM-metC-cntr-F | ACGCCAGAATCAAACCAATC | metC control primer | UK-1 |
STM-metC-cntr-R | ATCGCCAGGTAAGAATGACG | metC control primer | UK-1 |
STM-asd-TetA-SacB-F | GAACCACACGCAGGCCCGATAAGCGCTGCAATAGCCACTAATCAAAGGGAAAACTGTCCATATGC | Allelic exchange of asd with TetA-SacB | SL1344 |
STM-asd-TetA-SacB-R | CGCGCATACACAGCACATCTCTTTGCAGGAAAAAAACGCTTCCTAATTTTTGTTGACACTCTATC | Allelic exchange of asd with TetA-SacB | SL1344 |
STM-asd-rmvl-F | GAACCACACGCAGGCCCGATAAGCGCTGCAATAGCCACTAAGCGTTTTTTTCCTGCAAAGAGATGTGCTGTGTATGCGCG | Removal of TetA-SacB cassette | SL1344 |
STM-asd-rmvl-R | CGCGCATACACAGCACATCTCTTTGCAGGAAAAAAACGCTTAGTGGCTATTGCAGCGCTTATCGGGCCTGCGTGTGGTTC | Removal of TetA-SacB cassette | SL1344 |
STM-asd-ctrl-Rv | TAAGCGCTGCAATAGCCACT | asd control primer | SL1344 |
STM-asd-ctrl-Fw | TTGCGACTTTGGCTGCTTTT | asd control primer | SL1344 |
STM-alrN-TetA-SacB-F | CCCAAGTGGACCGGTCGACGCCTTAGCCTGAATTAGGTTAATCAAAGGGAAAACTGTCCATATGC | Allelic exchange of alrN with TetA-SacB | SL1344 |
STM-alrN-TetA-SacB-R | CAACGTTTGCATAGCGCGCATAACTGATAAAGGAAGTGAATCCTAATTTTTGTTGACACTCTATC | Allelic exchange of alrN with TetA-SacB | SL1344 |
STM-alrN-rmvl-F | CCCAAGTGGACCGGTCGACGCCTTAGCCTGAATTAGGTTATTCACTTCCTTTATCAGTTATGCGCGCTATGCAAACGTTG | Removal of TetA-SacB cassette | SL1344 |
STM-alrN-rmvl-R | CAACGTTTGCATAGCGCGCATAACTGATAAAGGAAGTGAATAACCTAATTCAGGCTAAGGCGTCGACCGGTCCACTTGGG | Removal of TetA-SacB cassette | SL1344 |
STM-alrN-ctrl-Fw | GTTTGGCGGCATGATTTGGA | alrN control primer | SL1344 |
STM-alrN-ctrl-Rv | CACCTTAGGCTGGACGATGG | alrN control primer | SL1344 |
STM-alrP-TetA-SacB-F | GATGAGTAACTCTCCGTCATTCTTTTAACAAGGAATTCAATCCTAATTTTTGTTGACACTCTATC | Allelic exchange of alrP with TetA-SacB | SL1344 |
STM-alrP-TetA-SacB-R | CCGGATAAGCGCAAGCGCCACCCGGCCCGCCGCGTATTTAATCAAAGGGAAAACTGTCCATATGC | Allelic exchange of alrP with TetA-SacB | SL1344 |
STM-alrP-rmvl-F | GATGAGTAACTCTCCGTCATTCTTTTAACAAGGAATTCAATAAATACGCGGCGGGCCGGGTGGCGCTTGCGCTTATCCGG | Removal of TetA-SacB cassette | SL1344 |
STM-alrP-rmvl-R | CCGGATAAGCGCAAGCGCCACCCGGCCCGCCGCGTATTTATTGAATTCCTTGTTAAAAGAATGACGGAGAGTTACTCATC | Removal of TetA-SacB cassette | SL1344 |
STM-alrP-ctrl-Fw | GGTACGGTTCGTCTGACGTT | alrP control primer | SL1344 |
STM-alrP-ctrl-Rv | TATTACCGGATGACGGCGTG | alrP control primer | SL1344 |
STM-metC-TetA-SacB-F | TAGTTTAGACATCCAGACGGTTAAAATCAGGAAACGCAACTCCTAATTTTTGTTGACACTCTATC | Allelic exchange of metC with TetA-SacB | SL1344 |
STM-metC-TetA-SacB-R | CGGAAATTGTCTGCATATATGTCCATCCCCGGCAACTTTAATCAAAGGGAAAACTGTCCATATGC | Allelic exchange of metC with TetA-SacB | SL1344 |
STM-metC-rmvl-F | TAGTTTAGACATCCAGACGGTTAAAATCAGGAAACGCAACTAAAGTTGCCGGGGATGGACATATATGCAGACAATTTCCG | Removal of TetA-SacB cassette | SL1344 |
STM-metC-rmvl-R | CGGAAATTGTCTGCATATATGTCCATCCCCGGCAACTTTAGTTGCGTTTCCTGATTTTAACCGTCTGGATGTCTAAACTA | Removal of TetA-SacB cassette | SL1344 |
STM-metC-ctrl-Fw | GCCAGGGTGCAGATGGTTAT | metC control primer | SL1344 |
STM-metC-ctrl-Rv | GACGCAACAAACGCAGACTT | metC control primer | SL1344 |
STM-asd-TetRA-Fw | GCGCGCATACACAGCACATCTCTTTGCAGGAAAAAAACGCTTTAAGACCCACTTTCACATT | Allelic exchange of asd with TetRA | SL1344 |
STM-asd-TetRA-Rv | AACCACACGCAGGCCCGATAAGCGCTGCAATAGCCACTACTAAGCACTTGTCTCCTG | Allelic exchange of asd with TetRA | SL1344 |
