Table 3 Primers used for antibody repertoire sequencing.
From: Uncoupling of invasive bacterial mucosal immunogenicity from pathogenicity
IgH Forward Mix | 5′ → 3′ sequence Illumina Adapter sequence read 1 + Diversity region + VH 5′-specific region |
|---|---|
IgH-UAd-fw1 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGAKGTRMAGCTTCAGGAGTC |
IgH-UAd-fw2 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGAGGTBCAGCTBCAGCAGTC |
IgH-UAd-fw3 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNCAGGTGCAGCTGAAGSASTC |
IgH-UAd-fw4 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGAGGTCCARCTGCAACARTC |
IgH-UAd-fw5 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNCAGGTYCAGCTBCAGCARTC |
IgH-UAd-fw6 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNCAGGTYCARCTGCAGCAGTC |
IgH-UAd-fw7 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNCAGGTCCACGTGAAGCAGTC |
IgH-UAd-fw8 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGAGGTGAASSTGGTGGAATC |
IgH-UAd-fw9 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGAVGTGAWGYTGGTGGAGTC |
IgH-UAd-fw10 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGAGGTGCAGSKGGTGGAGTC |
IgH-UAd-fw11 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGAKGTGCAMCTGGTGGAGTC |
IgH-UAd-fw12 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGAGGTGAAGCTGATGGARTC |
IgH-UAd-fw13 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGAGGTGCARCTTGTTGAGTC |
IgH-UAd-fw14 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGARGTRAAGCTTCTCGAGTC |
IgH-UAd-fw15 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGAAGTGAARSTTGAGGAGTC |
IgH-UAd-fw16 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNCAGGTTACTCTRAAAGWGTSTG |
IgH-UAd-fw17 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNCAGGTCCAACTVCAGCARCC |
IgH-UAd-fw18 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGATGTGAACTTGGAAGTGTC |
IgH-UAd-fw19 | TCGTCGGCAGCGTCAGATGTGTATAAGAGACAGNNNNGAGGTGAAGGTCATCGAGTC |
IgH Reverse Primer | 5′ → 3′ sequence Illumina Adapter sequence read 2 + Diversity region + IgA constant region specific |
|---|---|
IgA-const-rev | GTCTCGTGGGCTCGGAGATGTGTATAAGAGACANNNNGAGCTCGTGGGAGTGTCAGTG |
