Fig. 1: SYAC1 expression in Arabidopsis and in response to hormonal treatments.

a, b Expression of SYAC1 in 5-day-old Arabidopsis roots analyzed by RT-qPCR. Seedlings were treated with cytokinin (10 µM) and auxin (1 µM) and both hormones together for 3 h (a) or both hormones together for indicated time intervals (b). Significant differences to mock treated roots are indicated as ***P < 0.001 (t-test, n = 5–8 biological replicates with three technical replicates each, average ± SE). c–i SYAC1 expression monitored using pSYAC1:GUS reporter. Roots treated with cytokinin (10 µM) and auxin (1 µM) and both hormones together for 6 h (c), and untreated mature embryo (d), 2-, 3- and 4-day-old seedling (e–g); 8-week-old shoot (h), and dark grown hypocotyl and apical hook of 3-day-old seedling (i). Scale bar 50 µm (c, e–g), 200 µm (d), 500 µm (h), and 100 µm (i). 1-Naphthaleneacetic acid and N6-benzyladenine used as auxin and cytokinin, respectively.