Fig. 2: Impact of modulated SYAC1 expression on seedling growth and sensitivity to plant hormones. | Nature Communications

Fig. 2: Impact of modulated SYAC1 expression on seedling growth and sensitivity to plant hormones.

From: SYNERGISTIC ON AUXIN AND CYTOKININ 1 positively regulates growth and attenuates soil pathogen resistance

Fig. 2

a Real time monitoring of formation (F), maintenance (M), and opening (O) phase during apical hook development in wild type, syac1-3, syac1-5, SYAC1ox overexpressor, and pSYAC1:gSYAC1-GFP syac1-3 seedlings. syac1-3 and syac1-5 exhibit premature transition from M to O phase when compared with wild-type control (black arrow indicates time of transition from M to O in syac1) and SYAC1ox fails to form an apical hook (n = 9–11; average ± SE). Analyses of hypocotyl (b) and root (c) length in 4-day-old seedlings grown in darkness (n = 14–20; ±SE) (b) and 5-day-old seedlings grown on the light (n = 10; ±SE) (c). Analysis of root sensitivity to long-term (d) and transient (e) treatments with hormones. Wild-type and syac1 mutant seedlings were grown for 5 days on cytokinin (10 µM), auxin (1 µM), and on both hormones together (n = 10; average ± SE) (d) or 5-day-old seedlings were transferred for 6 h on hormone free or with auxin (0.05 µM), cytokinin (0.1 µM), or auxin plus cytokinin supplemented media (n = 10–20) (e). Wild type I and III represent respective control to syac1-3 and SYAC1-GFPox lines, respectively. Wild type II was isolated from syac1-5 heterozygote population (b–e). In the boxplots, center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by Origin software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, individual data points are represented by dots (e). f Transient induction of SYAC1 expression by estradiol (EST) triggers rapid deceleration of root growth. Five-day-old pEST:SYAC1 and wild-type seedlings transferred to mock or EST containing medium (n = 9–10; average ± SE). Significant differences are indicated as *P < 0.05, **P < 0.01, and ***P < 0.001 (t-test) (b–f), compared with mock treated control (b, c, f) or to respective treatment of wild type (d, e). 1-Naphthaleneacetic acid and N6-benzyladenine used as auxin and cytokinin, respectively.

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