Fig. 3: SYAC1 colocalizes with Golgi/TGN/endosomal/PVC markers.

Co-imunolocalization of SYAC1-GFP with markers for different subcellular compartments (a) and quantification of colocalization using Pearson correlation coefficient (b). Five-day-old pEST:SYAC1-GFP seedlings grown on mock medium treated with 5 µM estradiol for 6 h used in co-imunolocalization experiments (n = 10 roots with 1–5 epidermal cells each). Co-imunolocalization performed using antibodies against anti-GFP and subcellular markers. In the boxplots, center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by Origin software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles, individual data points are represented by dots. ER Endoplasmic reticulum, TGN trans-Golgi network, PVC prevacuolar compartment, PM plasma membrane. Scale bar 5 µm.