Fig. 4: SYAC1 interacts with YIP4a, YIP4b, YIP5b, and ECH protein. | Nature Communications

Fig. 4: SYAC1 interacts with YIP4a, YIP4b, YIP5b, and ECH protein.

From: SYNERGISTIC ON AUXIN AND CYTOKININ 1 positively regulates growth and attenuates soil pathogen resistance

Fig. 4

a Y2H assay confirms SYAC1 (prey, cloned in pDEST-GADT7) interaction with YIP4a, YIP4b, YIP5b, and ECH. Weaker interaction detected for KCR1 and DSK2, whereas no interaction found between SYAC1 with PHB4 protein. Yeast cells were grown on SD-LWH minimal media without histidin (H), leucin (L) and tryptophan (W), supplemented with 3-amino-1,2,4-triazole (3AT). Empty pDEST-GBKT7 (bait) vector was used as negative control. b Bimolecular fluorescence complementation (BiFC) assay in Arabidopsis root cell culture protoplasts reveals interaction between SYAC1 and YIP4a, YIP4b YIP5b, ECH, no interaction detected with KCR1, and PHB4 proteins. SYAC1-GFP and SYAC1-cEYFP (C-terminal part of EYFP) were used as a positive and negative control, respectively. Scale bar 10 µm. c Co-immunoprecipitation (Co-IP) assay of SYAC1-GFP with ECH-HA and SYAC1-HA with YIP4a-MYC transiently expressed in Arabidopsis root cell culture protoplasts. The anti-GFP and anti-MYC antibodies immunoprecipitates were analyzed in a western blot assay with anti-HA antibodies.

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