Fig. 6: Loss of SYAC1 activity increases tolerance to Plasmodiophora brassicae infection.

a syac1-3 and syac1-5 mutant alleles exhibit reduced sensitivity to fungal infection when compared with wild-type control. In contrast, the overexpression of SYAC1ox results in oversensitivity to the pathogen. Five-scale classification was used to evaluate disease severity: 0 (no symptoms), 1 (very small galls mainly on lateral roots and that do not impair the main root), 2 (small galls covering the main root and few lateral roots), 3 (medium to large galls, also including the main root; plant growth might be impaired), and 4 (severe galls on lateral root, main root, or rosette; fine roots completely destroyed; plant growth is reduced). Inoculation was performed with 10⁴, 10⁵, and 10⁶ spore concentration. Significant differences between datasets are indicated by different letters. Data were statistically analyzed using the Kruskal–Wallis test.