Fig. 4: Down-regulation of lipid metabolism in injured murine but not human nerves.

a Alterations in GO (Gene Ontology) terms in murine/human nerves 24 h upon injury. b Fold change expression of lipid metabolism related genes in murine/human nerves after injury compared to 0 h. Significantly changed values are depicted in bold. c–j qPCR validation of selected lipogenic genes at different time points post injury (TPI). Bars show mean with SEM. Grey or red asterisks indicate significance compared to 0 h time point for murine or human samples respectively. Blue asterisks indicate significant differences between mouse and human for the particular time point. k, l Down-regulation of PPARg⁺ in SCs of teased nerve fibres. Scale bar is 10 µm. Bars in l show mean with SD (P = 0.0286). m qPCR of selected lipogenic genes in nerves injured in vivo. Graph shows mean with SEM for each time point. n, o Transcription factor (TF) binding motif analysis for genes significantly down-regulated at least threefold in murine (n) or human (o) nerves 24 h after injury. P-values were calculated by Pscan Ver. 1.5 software using a two-tailed Z-test. p qPCR validation of expression of the gene Medag in human/murine nerves at different time points post injury. Analysed biological replicates: for (a, b, n–o) human n = 5, murine n = 3 for each time point, for (c–h) n = 15, 15, 15 and 12 for human and 10, 10, 10 and 4 for mouse at 0 h, 2 h, 24 h and 48 h respectively, for (i, j, p) n = 7, 7, 7 and 7 for human and 10, 10, 10 and 4 for mouse at 0 h, 2 h, 24 h and 48 h respectively, for l n = 4 for each time point, for m n = 9, 3 and 3 at 0 h, 2 h and 24 h respectively. Two-sided Mann–Whitney test was used to calculate statistical significance (*P < 0.05, **P < 0.005, ***P < 0.001). Source data are provided as a Source Data file.