Fig. 3: Chronic stress selectively increases prefrontal cortical glutamate release onto dmPFC→BLA PNs.
From: Identification of a prefrontal cortex-to-amygdala pathway for chronic stress-induced anxiety
a Representative traces showing EPSCs in dmPFC↔BLA PNs upon paired light stimuli of dmPFC inputs (separated by 100 ms). Scale bar = 50 ms, 50 pA. b Summary plots of paired pulse ratio (PPR) in dmPFC↔BLA PNs. Control, n = 13 neurons/5 mice; CRS, n = 14 neurons/5 mice. c Same as in a except that the data were from dmPFC→BLA PNs. Scale bar = 50 ms, 50 pA. d Summary plots of PPR in dmPFC→BLA PNs. Control, n = 14 neurons/5 mice; CRS, n = 13 neurons/5 mice. e Representative traces showing the NMDA current in dmPFC↔BLA PNs in response to the 1st, 10th, 20th, 40th, 60th, 90th, and 120th photostimulation during MK-801 perfusion. Scale bar = 50 ms, 100 pA. f The progressive blockade of NMDA currents by MK-801 during light activation of dmPFC inputs. Each point represented averaged currents normalized to the first one. Inset showing comparison of the averaged τ values. Control, n = 9 neurons/4 mice; CRS, n = 10 neurons/4 mice. g Same as in e except that the data were from dmPFC→BLA PNs. Scale bar = 50 ms, 100 pA. h Same as in f except that the data were from dmPFC→BLA PNs. Control, n = 10 neurons/4 mice; CRS, n = 9 neurons/4 mice. Data are presented as mean ± SEM. Statistics are shown in Supplementary Table 1. n.s., not significant; *p < 0.05, **p < 0.01. Source data are provided as a Source Data file.
