Fig. 6: Activation of dmPFC inputs reverses CRS-induced increase of glutamate release in dmPFC synapses terminating on dmPFC→BLA PNs.

a Schematic showing the experimental procedures for ex vivo light stimulation (LS) and PPR recording. b Representative traces showing paired EPSCs in dmPFC↔BLA and dmPFC→BLA PNs of CRS mice prior and posterior to 3-min LS. Two light pulses were delivered at an interval of 100 ms. Scale bar = 50 ms, 50 pA. c Effect of 3-min LS on the PPR in the two PN populations of CRS mice. dmPFC↔BLA PNs, n = 9 neurons/6 mice; dmPFC→BLA PNs, n = 10 neurons/7 mice. d Same as in b except that the LS duration was extended to 10 min. e Effect of 10-min LS on the PPR in the two PN populations of CRS mice. dmPFC↔BLA PNs, n = 9 neurons/5 mice; dmPFC→BLA PNs, n = 9 neurons/6 mice. f Schematic showing experimental procedures for in vivo LS in CRS mice. g Representative traces showing paired EPSCs in dmPFC↔BLA PNs and dmPFC→BLA PNs from unstimulated CRS mice and mice receiving in vivo LS for 3 or 10 min. Scale bar = 50 ms, 50 pA. h Summary plots of PPR in dmPFC↔BLA PNs. CRS, n = 14 neurons/4 mice; CRS + 3 min LS, n = 14 neurons/5 mice; CRS + 10 min LS, n = 13 neurons/4 mice. i Summary plots of PPR in dmPFC→BLA PNs. CRS, n = 16 neurons/5 mice; CRS + 3 min LS, n = 13 neurons/4 mice; CRS + 10 min LS, n = 16 neurons/5 mice. j Representative traces of AMPA and NMDA receptor currents recorded in the dmPFC↔BLA and dmPFC→BLA PNs. Scale bar = 50 ms, 80 pA. k Summary plot of AMPA/NMDA ratio in dmPFC↔BLA PNs. CRS, n = 15 neurons/5 mice; CRS + 3 min LS, n = 13 neurons/4 mice; CRS + 10 min LS, n = 13 neurons/4 mice. l Summary plot of AMPA/NMDA ratio in dmPFC→BLA PNs. CRS, n = 16 neurons/5 mice; CRS + 3 min LS, n = 15 neurons/5 mice; CRS + 10 min LS, n = 14 neurons/4 mice. Data are presented as mean ± SEM. Statistics are shown in Supplementary Table 1. *p < 0.05, **p < 0.01. Source data are provided as a Source Data file.