Fig. 6: Synapsis defects in Brme1^−⁄− spermatocytes.

a Population of meiotic substages in WT (2623 cells) and Brme1^−⁄− (2565 cells) pooled from three mice for each genotype. The mean values of three independent experiments from three different mice are shown. Leptotene (L), zygotene (Z), pachytene (P), diplotene (D), metaphase I (MI). Error bars: SD. b Immunostaining of Brme1^−⁄− zygotene spermatocytes. Magnified pictures with schematics highlight the partner switch. Graph: the ratio of zygotene spermatocytes (74 cells pooled from three mice for each genotypes) showing at least one partner switch. The mean values of three independent experiments from three different mice are shown. Error bars: SD. Scale bars: 5 μm or 1 μm in the magnified panel. c Immunostaining of pachytene spermatocytes. Magnified pictures with schematics highlight the sex chromosomes. Arrowheads: sex chromosome asynapsis. Graph: the frequency of cells with sex chromosome synapsis defects in WT (191 cells) and Brme1^−⁄− (285 cells) pooled from three mice for each genotype. The mean values of three independent experiments from three different mice are shown. Error bars: SD. Scale bars: 5 μm or 1 μm in the magnified panel. d Immunostaining of pachytene spermatocytes. Arrowheads: asynapsis. The magnified picture highlights the incompletely synapsed autosomes (juxtaposition) with a gap between aligned homologs (yellow arrowhead). Graph: the frequency of autosomal synapsis defects in WT (192 cells) and Brme1^−⁄− (280 cells) pooled from three mice for each genotype. The mean values of three independent experiments from three different mice are shown. Error bars: SD. Scale bars: 5 μm or 1 μm in the magnified panel. All analyses used two-tailed t tests. ns: not significant. *p < 0.05, **p < 0.01, ***p < 0.001. Source data are provided as a Source Data file.