Fig. 8: MEILB2 and BRME1 impair mitotic HR. | Nature Communications

Fig. 8: MEILB2 and BRME1 impair mitotic HR.

From: The BRCA2-MEILB2-BRME1 complex governs meiotic recombination and impairs the mitotic BRCA2-RAD51 function in cancer cells

Fig. 8

a Mouse B16-F1 cells transfected with MEILB2-MYC (WT) or MEILB2-R204T-MYC (R204T) stained with RAD51 and MYC antibodies. Graph: the number of MYC foci colocalizing with RAD51. n shows the analyzed RAD51-positive S to G2 phase cells. Bars: mean values with SD. b IPs with the MYC antibody from B16-F1 cells expressing MEILB2-MYC or MEILB2-R204T-MYC with GFP-BRCA2 truncations; C (a.a. 2036–3329) or MBD (a.a. 2117–2339). c Immunostaining of zygotene spermatocytes expressing GFP-MEILB2 or GFP-MEILB2-R204T. d Mouse B16-F1 cells transfected with FLAG-BRME1 (left), MEILB2-MYC (middle), or both FLAG-BRME1 and MEILB2-MYC (right) and stained with RAD51 and FLAG (for BRME1) or MYC (for MEILB2) antibodies. e Mouse B16-F1 cells with or without MMC treatment transfected with MEILB2-MYC (WT or R204T) or FLAG-BRME1 and stained with RAD51 or MYC (for MEILB2) and FLAG (for BRME1) antibodies and DAPI. Graph: the number of RAD51 foci per cell. Red bars: mean values. n shows the analyzed cell number pooled from three independent transfections. f Schematic of the U2OS-DSB reporter cell line. g U2OS-DSB reporter cell line with or without DSB induction transfected with MEILB2-MYC or FLAG-BRME1 and stained with the RAD51 antibody. Graph: the frequency of cells with RAD51 accumulation at the DSB. The analyzed cell numbers are 78, 70, and 68 cells for mock, MEILB2, and BRME1, respectively. The mean values of two independent experiments are shown. All analyses used two-tailed t tests. ns: not significant. ***p < 0.001, ****p < 0.0001. Scale bars: 5 μm or 1 μm in the magnified panel. Source data are provided as a Source Data file.

Back to article page