Fig. 3: Infiltration of CD11b⁺ Gr1⁺ immunosuppressive myeloid cells in mouse penile cancer.

a Frequency of CD45⁺ immune cells of all live cells in penile tissues from Cre^- littermates (control, n = 2) and SA mice (n = 7, 4–5 months of age), with data from CyTOF. b Frequency of indicated immune cell subpopulations of all immune cells for the same sets of penile samples as in a. c Representative viSNE plots of indicated markers for the same sets of penile samples as in a. Note that the sparse dots and numerous dots for Cre^- littermate or SA mouse, respectively, reflect the vast difference of the total intratumoral immune cell frequency of the two genotypes. d Activity of CD11b⁺ Gr1⁺ cells from SA tumors to suppress proliferation of normal spleen T cells stimulated with anti-CD3/CD28 antibodies. T cells were preloaded with CFSE whose signals declined as T cells divided. Higher MDSC: T cell ratio led to lower percentage of CFSE^low T cells (n = 4, 4, 6, and 3 for the ratios in order). e Representative viSNE plots of CD3e and PD1 (CD279) for SA tumors, showing the partial overlap of the two signals. In a, b, all data are shown with the line representing the median. In d, box plots visualize the five-number summary of a data set (minimum, lower quartile, median, upper quartile and maximum). *P < 0.05, **P < 0.01, ***P < 0.001, two-sided Student’s t test.