Fig. 5: Renal single-cell RNAseq expression of potential urine biomarkers in LN and healthy controls. | Nature Communications

Fig. 5: Renal single-cell RNAseq expression of potential urine biomarkers in LN and healthy controls.

From: Comprehensive aptamer-based screening identifies a spectrum of urinary biomarkers of lupus nephritis across ethnicities

Fig. 5

a Feature plots of potential urine biomarkers where each dot is a cell and the color intensity (gray = low, to red = high) indicates expression of the indicated gene within each cell, as deduced by RNAseq data analysis of 21 LN renal tissue samples. CCL2 = MCP-1; CAST = calpastatin; HPX = hemopexin; PRDX6 = peroxiredoxin-6; BCL2A1 = BFL-1; SELE = sE-selectin. b tSNE plot of 1624 cells obtained from the renal biopsy of 21 LN patients and 3 healthy controls. Clusters are derived from PCA and graph-based clustering analysis and color-coded based on identified cell type. c Student’s t-test comparison of expression of CCL2 (MCP-1), VCAM-1, CAST (calpastatin), and FCG2RB between LN patients (N = 21) and healthy controls (N = 3) within the single-cell RNAseq dataset. The ends of the box represent the upper and lower quartiles, so the box spans the interquartile range. The median is marked by a horizontal line inside the box. The whiskers are the two lines outside the box that indicate 1.5 times the interquartile range from the upper or lower quartile.

Back to article page