Fig. 4: 3D-EMISH statistical analysis of chromatin-folding domains.

a Classified 1, 2, 3, 4 distinctive chromatin-folding domains examples. Identified chromatin-folding domains in each 3D image were presented by different colors (magenta, green, cyan, and yellow for first, second, third, and fourth domain group, respectively); scale bar, 500 nm. b 3D-EMISH chromatin-folding domain histogram of 229 structures: one domain (58/229, 25%), two domains (90/229, 39%), three domains (70/229, 31%), four domains (11/229, 5%). Volume (c), surface area (d), and form factor (e) statistics for all 229 images for four distinctive classified chromatin-folding domains. This experiment was repeated independently two times with similar results. Error bars indicate SEM. Kruskal–Wallis test, and two-sided Mann–Whitney tests with Bonferroni correction were performed; ns (statistically insignificant); statistically significant differences: *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001. P-values [volume] (1~2: 0.007; 1~3: 0.000; 1~4: 0.000; 2~3: 0.166; 2~4: 0.009; 3~4: 0.261); [surface area] (1~2: 0.000; 1~3: 0.000; 1~4: 0.000; 2~3: 0.002; 2~4: 0.000; 3~4: 0.016); [form factor] (1~2: 0.025; 1~3: 0.000; 1~4: 0.000; 2~3: 0.000; 2~4: 0.000; 3~4: 0.007). Source data are provided as a Source Data file. Data distributions are shown in Supplementary Fig. 8.