Fig. 3: Regulation of Kng2 expression and BAT activity in BN/Ka rats.

a mRNA expression of K-kininogen (Kng2) in iBAT from 12 weeks old BN and BN/Ka rats at thermoneutrality (27 °C) and after 24 h or 22 days exposure to cold (4 °C) (n = 4 animals). b HMW-KNG2 protein expression in plasma and quantification in BN and BN/Ka rats under thermoneutral conditions (n = 3 animals). c Optical microscopy of H&E-stained iBAT from BN and BN/Ka rats under thermoneutral conditions (n = 4 animals). d UCP1 protein expression and quantification of UCP1 protein levels, expressed per tissue and total protein (below), in iBAT from BN and BN/Ka rats under thermoneutral conditions (n = 4 animals). e Representative infrared thermographies (left) and quantification of iBAT temperature (right) in BN and BN/Ka rats under thermoneutral conditions (n = 4 animals). ND, not detectable. Data are presented as means ± s.e.m. (bars). *P < 0.05 and ***P < 0.001 versus BN; ^#P < 0.05 versus thermoneutrality. P-values determined by two-tailed unpaired Student’s t-test (for genotypes) and two ways ANOVA with Tukey’s post hoc test (for temperature) (a) and two-tailed unpaired Student’s t-test (b, d, e). Source data are provided as a Source data file.