Fig. 5: Effects of local pharmacological inhibition of B1/B2 receptors at iBAT and iWAT.

a H&E-stained iBAT histological sections and quantification (at least 3 different pictures) of cell size in 2 months old WT C57BL/6J mice exposed to 4 °C for 1 week and then implanted in the back with mini-pumps delivering either PBS or a cocktail of B1 and B2 antagonists; iBAT weight normalized to tibia length (n = 8 animals for PBS and n = 7 animals for antagonists). b Quantification of total protein per milligram tissue and in the entire iBAT depot (n = 8 animals for PBS and n = 7 animals for antagonists). c UCP1 protein expression in iBAT, with quantification (n = 7 animals for PBS and n = 6 animals for antagonists). d H&E-stained iWAT histological sections and quantification of browning area (at least three different pictures) in 2 months old WT C57BL/6 J mice exposed to 4 °C for 1 week and implanted in the leg with mini-pumps delivering either PBS or a cocktail of B1 and B2 antagonists; iWAT weight normalized to tibia length (n = 5 animals for PBS and n = 6 animals for antagonists). e Immunofluorescence detection of UCP1 protein in iWAT histological sections, with quantification (at least three different pictures). Data are presented as means ± s.e.m. (bars). *P < 0.05, **P < 0.01, and ***P < 0.001 versus corresponding controls; two-tailed unpaired Student’s t-test. a–c) n = 7; d, e) n = 6. Source data are provided as a Source data file.