Fig. 3: Impaired production Of CD104⁺CCL21⁺ mTEC^low In LTβR^TEC mice.

a mTEC^low from Foxn1^Cre and LTβR^TEC mice, analysed for surface expression of CD104 and intracellular expression of CCL21. Bar graphs show cell percentages and absolute cell numbers in Foxn1^Cre (n = 6 biologically independent samples, closed symbols) and LTβR^TEC mice (n = 6 biologically independent samples, open symbols), over three independent experiments. Significant P values using two-tailed unpaired t test are as follows: no. of cells P = 0.0009. b MFI levels of intracellular CCL21 expression in pre-gated CD104⁺CCL21⁺ mTEC^low from Foxn1^Cre (closed symbols) and LTβR^TEC (open symbols) mice, n = 6 biologically independent samples, over three independent experiments. c Levels of Ccl21 mRNA in FACS sorted CD104⁺ mTEC^low from Foxn1^Cre (closed symbols) and LTβR^TEC mice (open symbols), data representative of three biological sorts. d Alymphoid 2dGuo-treated FTOC cultured for 4 days in the presence or absence of agonistic anti-LTβR (2 μg/ml) were pooled and analysed by flow cytometry for the expression of CCL21 and CD104. Freshly isolated adult WT mTEC^low were stained alongside for comparison. Significant P values using two-tailed unpaired t test are as follows: % cells P = 0.0003, no. of cells P = 0.0102. Six FTOC cultured lobes were pooled per data point, n = 3 biologically independent samples, over three independent experiments. All data are represented as mean ± SEM. *P < 0.05 and ***P < 0.001. Source data are provided as a Source Data file.