Fig. 2: Association between F. prausnitzii, systemic concentrations of SCFA and clinical outcomes.
From: Systemic short chain fatty acids limit antitumor effect of CTLA-4 blockade in hosts with cancer
a Quantity Mean 16S/ng DNA of F. prausnitzii and B. fragilis at baseline (Q-PCR). Each patient’s microbiota is presented in the graph (n = 38). 0–6 M: overall survival ranging from 0 to 6 months (n = 4), >6–18 M: overall survival ranging from 6 to 18 months (n = 23), >18 M: overall survival >18 months (n = 11). Two-Way ANOVA followed by Tukey’s tests for multiple comparisons was performed. p-values are indicated on each graph; ns, not significant. b Kaplan–Meier survival curves of PFS of patients classified into two groups according to median of F. prausnitzii/ng DNA (Low vs high F. prau; left panel) and 16S BACTERIA/ ng DNA (low vs high Bacteria; right panel). Date were performed using Q-PCR analyses on feces at baseline (n = 38), Log-rank (mantel-cox) test was used c Spearman correlation between the relative abundance of Firmicutes assessed by 16S DNA analysis (% of total reads NGS) and serum butyrate at baseline (n = 33). d Q-PCR analyses on feces at baseline of F. prausnitzii (16S F.prau/ng DNA) according to the median concentrations of serum acetate, propionate and butyrate (n = 33). e As in (d) but proportion (n = 33). Each dot represents a mean quantity (assessed in duplicate for each sample) for one patient. Two-tailed Mann–Whitney tests were used (d, e). p-values are indicated on each graph; ns, not significant. Kaplan–Meier survival curves of PFS according to the median value of serum propionate (left panels) and butyrate (right panels) concentrations in French (f, n = 40) and Italian (g, n = 45) cohort, Log-rank (mantel-cox) test was used. h Pearson correlations between serum concentrations of acetate, butyrate and propionate and PFS (n = 40) in French cohort; i As in (h) but for OS (n = 40) in French cohort. Each dot represents one patient. p and r are indicated on each graph.
