Fig. 6: Nrd1CID–Set1Δ200 fusion does not bypass the role of Paf1C and H2Bub in H3K4me.
From: The Set1 N-terminal domain and Swd2 interact with RNA polymerase II CTD to recruit COMPASS
a Wild-type Set1 (Set1), Set1Δ200 (SΔ200), and Nrd1CID–Set1Δ200 (NSΔ200) were transformed into set1Δ, set1Δpaf1Δ, or set1Δrtf1Δ cells. Protein levels and histone methylations in whole-cell extracts were analyzed by immunoblotting. TBP and Histone H3 were used as loading controls. Parallel experiments in set1Δbre1Δ cells showed the same loss of H3K4 methylation (Supplemental Fig. 6a). b Set1 association with RNApII was quantitated by immunoprecipitating Set1 using α-FLAG beads and probing for Rpb1 CTD Ser5P and Set1. Set1 and Ser5P signal intensities from each immunoblot were quantitated using ImageJ, and Ser5P signals were normalized to those for Set1. Circles show individual experimental values, and error bars show s.e.m. (n = 5). Values from wild type Set1 transformants were normalized to 1.0 for each experiment. Source data are provided as a Source data file.
