Fig. 3: Microsporidia MB is maternally transmitted and does not bias sex ratio.
From: A microsporidian impairs Plasmodium falciparum transmission in Anopheles arabiensis mosquitoes
a The vertical transmission efficiency of Microsporidia MB to G1 depends on the G0 (maternal) Microsporidia MB intensity. In a linear regression, the slope is significantly non-zero (P = 0.02, F = 6.169, and n = 27). b The intensity of Microsporidia MB in G1 offspring is correlated to G0 maternal intensity, with a linear regression the slope that is significantly non-zero (P = 0.01, F = 6.98 and n = 27). c Fluorescence microscopy with a Microsporidia MB specific FISH probe (MB probe) indicates that Microsporidia MB (a) is localized to the posterior of developing vitellogenic egg chambers (b) in An. arabiensis. Scale bar, 20 μm. Representative images as observed in three independent experiments. d The sex ratio of broods from Microsporidia MB infected mothers does not differ significantly from Microsporidia MB uninfected mothers suggesting there is no sex ratio distortion (Unpaired two-tailed t-test, P = 0.32, t = 1.02, df=13). Dashed line reflects overall sex ratio (Uninfected control = 100:108 or 52% female, Microsporidia MB = 56:64 or 54% female). Column widths reflect sample sizes Source data are provided as a Source Data file.
