Fig. 4: Oncogene expression induces basal epithelial cell extrusion in the accessory gland. | Nature Communications

Fig. 4: Oncogene expression induces basal epithelial cell extrusion in the accessory gland.

From: Sequential Ras/MAPK and PI3K/AKT/mTOR pathways recruitment drives basal extrusion in the prostate-like gland of Drosophila

Fig. 4

a, b GFP-RasV12 and (c–f) RFP-RasV12 expressing clones are able to migrate from the epithelial compartment. a Alexa633-phalloidin (Phalloidin) staining reveals disorganisation of the muscle fibres at the site of extrusion of GFP-RasV12 expressing clones (white arrow). b Zoomed image of (a) reveals that the tumour is still anchored to the epithelium (white arrow). c, j Alexa633-phalloidin (Phalloidin) staining reveals the muscle fibres (c–f, i, j) as Viking-GFP staining reveals basement membranes (c–h). (d, f, h, j): optical cross-sections of top panels (respectively, c, e, g, i). Staining reveals that RFP-RasV12 tumour develops outside the epithelial compartment, as it is not enclosed by a basement membrane or a muscle layer (yellow arrows). Basement membrane staining reveals tracheal branches inside the tumour (white arrows). DAPI (blue) reveals nuclei in (a–j). Representative images from two experiments. Scale bars: 50 μm.

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