Fig. 1: AR signalling is conserved in ARI-resistant cells.
From: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
a Representative pictures of WT and ARI-resistant LNCaP cells cultured in 2D conditions. Scale bar represents 100 µm. b Representative pictures of WT and ARI-resistant LNCaP organoids embedded in Matrigel. Scale bar represents 50 µm. c Quantification of cell (top panel) and organoid (bottom panel) diameter. d Cell proliferation of WT and ARI-resistant LNCaP cells after 48 and 72 h. Cell count is normalised to initial number of cells at T0. e Cell proliferation of WT and ARI-resistant LNCaP cells treated for 48 h with different AR inhibitors (10 µM). Cell count is normalised to non-treated condition. f Western blot analysis of AR, KLK3 and FKBP5 expression in WT and ARI-resistant LNCaP cells. HSC70 was used as a sample loading control. g RT-qPCR analysis of AR (full length, fl), KLK3 and FKBP5 expression in WT and ARI-resistant LNCaP cells. CASC3 was used as a normalising control. h Immunofluorescence showing nuclear AR expression in WT and ARI-resistant LNCaP cells. Scale bar represents 20 µm. c–e, g Data are presented as mean values +/− SD. c, g *p-value < 0.05 using a 1-way ANOVA with a Dunnett’s multiple comparisons test. d, e *p-value < 0.05 using a 2-way ANOVA with a Tukey’s multiple comparisons test. Source data are provided as a Source Data File.
