Fig. 3: Lipid metabolism is strongly dysregulated in ARI-resistant cells.
From: 2,4-dienoyl-CoA reductase regulates lipid homeostasis in treatment-resistant prostate cancer
a Labelled palmitate fraction derived from 13C-glucose (left panel) and relative isotopologue distribution of palmitic acid in ARI-resistant and WT LNCaP cells following 13C-glucose incubation for 72 h (right panel). b Western blot analysis of ACC, ACLY, and phospho-ACLY expression in WT and ARI-resistant LNCaP cells. HSC70 is used as a sample loading control. c Heatmap illustrating the steady-state levels of significantly regulated lipids in ARI-resistant cells when compared to WT LNCaP (FC > 1.5, p < 0.05 using a 1-way ANOVA with a Dunnett’s multiple comparisons test). Values are expressed as log(FC). a Data are presented as mean values +/− SD. a *p-value < 0.05 using a 1-way ANOVA with a Dunnett’s multiple comparisons test. Cer ceramide, CL cardiolipin, DG diacylglycerol, LysoPC lysophosphatidylcholine, PC phosphatidylcholine, PE phosphatidylethanolamine, PS phosphatidylserine, SM sphingomyelin, TG triglyceride. Source data are provided as a Source data File.
