Fig. 4: The performance of three basic optogenetics tools.
From: Light-powered Escherichia coli cell division for chemical production
a–c The schematic diagram of BLAT (blue-light), BLRT (blue-light), and NRAT (NIR-light), respectively. Panels a and b are adapted from Jayaraman et al.38. d–f Fluorescence activation with BLAT, BLRT, and NRAT under dark, 0.8 W/cm2 blue-light, and NIR-light, respectively. g–i Blue-light or NIR-light LEDs were mounted on the bottom of a photomask with a setup for pattern illumination. Red, green, blue, and chinese panda-patterned masks made from aluminum foil were placed on top of the photomask to illuminate culture plate. In this setup, photomasks were placed onto the bottom of the engineered E. coli-culture plate with BLAT, BLRT, and NRAT, respectively. PJ23119: constitutive promoter; Plux: quorum sensing promoter; PmrkA: MrkH-targeted promoter; EL222: light-sensitive protein from Erythrobacter litoralis; bphS: a c-di-GMP diguanylate cyclase from Rhodobacter sphaeroides; bphO: a heme oxygenase from Rhodobacter sphaeroides; yhjH: c-di-GMP PDE from E. coli; mrkH: a transcriptional factor from Klebsiella pneumoniae. The box plots define the minima, maxima, center, and bounds of box. d–f n values are shown as mean ± s.d. from three (n = 3) biological independent replicates. Source data underlying Fig. 4d–i are provided as a Source data file.
