Fig. 2: hnRNP H/F subcellular localization and association with polysomal fractions. | Nature Communications

Fig. 2: hnRNP H/F subcellular localization and association with polysomal fractions.

From: hnRNP H/F drive RNA G-quadruplex-mediated translation linked to genomic instability and therapy resistance in glioblastoma

Fig. 2

a Subcellular fractionation of GBM cell lines, followed by western blot analysis of hnRNP H/F, eIF4A (cytosolic and microsomal marker), PERK (microsomal marker), histone H3 (nuclear marker) and tubulin (cytosolic marker associated to microsomes). Nuclear (N), microsomal (M), and cytosolic fractions (C). Shown is a representative result from n = 2 independent experiments. Source data are provided as a Source Data file. b Polysome profile of U251 cells untreated (NT) or puromycin treated (Puro), followed by western blot analysis from individual non-polysomal (NP) and polysomal (P) fractions by probing for hnRNP H/F, DHX36, DHX9, DDX3X, eIF4A. EEA1: negative control. RPS6: positive control. Shown is a representative result from n = 3 independent experiments. Source data are provided as a Source Data file. c As in b, except that cells were NT or treated with 20 μM carboxypyridostatin (cPDS) for 1 h, and probing for hnRNP H/F or RPL22 (negative control). Shown is a representative result from n = 2 independent experiments. Source data are provided as a Source Data file. d Repartition of hnRNP H/F proteins in polysomal fractions was quantified with n = 2 independent experiments.

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