Fig. 5: HA-FMRP(1–297)-tat immunolabel persists at least 24 h in Fmr1 KO mice.
From: FMRP(1–297)-tat restores ion channel and synaptic function in a model of Fragile X syndrome
a, b Qualitative comparison between HA immunolabel intensity in cerebellar tissue sections (a) or neocortex (b) prepared at the indicated times following tail vein injection of vehicle or 1.0 mg/kg HA-FMRP(1–297)-tat into P30–P60 Fmr1 KO mice. All sections were reacted simultaneously with anti-HA antibody and imaged/processed at identical camera settings and light intensities to enable qualitative comparisons. Arrows identify the position of representative cells positive for HA immunolabel. c CoIP of HA-FMRP(1–297)-tat with Cav3.1 and Kv4.3 in brain lysates of Fmr1 KO mice 5 h after tail vein injection of HA-FMRP(1–297)-tat. IP was conducted using a mouse anti-HA antibody and mouse normal IgG (as control) and immunoblotting using rabbit anti-Cav3.1 and rabbit anti-Kv4.3 antibodies after an injection of 27 mg/kg HA-FMRP(1–297)-tat. All immunolabel data in (a, b) and coIP tests in (c) were derived from at least three different animals. Dashed lines in (a) denote cell layer boundaries. Scale bar for (a, b), 20 µm. PC Purkinje cell layer, GC granule cell layer, mol molecular layer. Source data are provided as a Source Data file.
