Fig. 3: TFEB overexpression inhibits hepatocyte differentiation in vivo.
From: TFEB regulates murine liver cell fate during development and regeneration
a KEGG pathways enriched in upregulated and downregulated genes of P9 liver overexpressing TFEB compared to CTRL. b Heat map showing the relative fold change of hepatocyte and biliary genes. c RT-PCR analysis of hepatocyte- and cholangiocyte-specific markers in liver isolated from P0 and P9 mice (n = 3 biological replicates). The dashed line represents gene expression levels in CTRL liver. Data are represented as mean ± SEM. *p ≤ 0.05, **p ≤ 0.01 two-tailed Student’s t-test compared to age-matched CTRL. d Immunoblotting analysis of TFEB, HNF4α, and SOX9 in liver extracts from mice at P9. Actin was used as a loading control. e Left panel: Representative images of liver sections from P0 and P9 mice stained for CK19. Scale bar 50 μm. Right panels: triple immunostaining for HNF4α (red), SOX9 (green), and CK19 (white) in liver sections from P0 and P9 mice of the indicated genotype showing the hybrid characteristics of hepatocytes and cholangiocytes in Tg mice. Note the cells expressing SOX9 and CK19 (box1), SOX9 and HNF4α (box2) or SOX9/HNF4α/CK19 (box3). Scale bar 20 μm. Source data are provided as a Source Data file.
