Fig. 4: The identity of residues 36 and 37 in tRNA-Arg-CCU play a key role in m3C formation by METTL2A/2B in vitro. | Nature Communications

Fig. 4: The identity of residues 36 and 37 in tRNA-Arg-CCU play a key role in m3C formation by METTL2A/2B in vitro.

From: DALRD3 encodes a protein mutated in epileptic encephalopathy that targets arginine tRNAs for 3-methylcytosine modification

Fig. 4

a Anticodon loops of human tRNA-Arginine isoacceptors and known modifications. b Anticodon loops of in vitro transcribed tRNA-Arg-CCU and variants used in c, d. c, d Primer extension analysis of tRNA-Arg-CCU and variants incubated with either water, vector control eluate, purified METTL2A or METTL2B co-expressed with DALRD3. Primer length, 23 nt. e Quantification of m3C formation in d. Primer extension analysis was performed greater than three times for c and repeated three times for d. Bars represent the standard deviation from the mean. Statistical analysis was performed using one-way ANOVA and significance calculated using Tukey’s multiple comparison test. **P < 0.01. P = 0.0034 for WT versus C32A, 0.0039 for WT versus U36G, 0.0037 for WT versus A37G. Source data are provided as a Source data file.

Back to article page