Fig. 10: The mitochondria-associated protein Rab32 mediates the Dosmit-induced enlargement of mitochondria.
Mitochondria from: a, e Mef2/+ control flies, b flies with ectopic expression of wild-type Rab32, c Rab321/Rab321 flies, f Mef2 > Dosmit flies (in which Dosmit was ectopically expressed), g Mef2 > Dosmit + YFP-Rab32WT flies (in which both Dosmit and wild-type Rab32 were ectopically expressed), h Mef2 > Dosmit + YFP-Rab32CA flies in which both Dosmit and the constitutively active GTP-binding Rab32 mutant (Rab32CA) were ectopically expressed, i Mef2 > Dosmit + YFP-Rab32DN flies in which both Dosmit and the dominant-negative form of Rab32 (Rab32DN) were ectopically expressed, and j Rab321/Rab321; Mef2 > Dosmit flies in which Dosmit was ectopically expressed in the Rab32-null mutant background. d Distribution of size classes of mitochondria in the various genetic backgrounds. N = 62, 124, and 115 from left to right bars. Statistical test: Chi-Square Test (*p < 0.05). Green channel shows ATP5A staining; red channel shows phalloidin staining of F-actin in muscle. Scale bars: 10 μm. k Distribution of size classes of mitochondria in the various genetic backgrounds. N = 146, 43, 34, 26, 126, and 131 from left to right bars. Statistical test: Chi-Square Test (****p < 0.0001; ns: p = 0.0673). l–r TEM images of mitochondria from wild-type, Rab321/Rab321, Mef2 > Dosmit (Dsm), Mef2 > Dosmit + Rab32WT, Mef2 > Dosmit + Rab32CA, Mef2 > Dosmit + Rab32DN, and Rab321/Rab321; Mef2>Dosmit muscle tissue. Scale bars: 2 μm. s Number of vesicles per unit area for 1-week-old flies from panels l–r (mean ± SD). N = 8, 10, 7, 3, 3, 9, and 6 from left to right bars. Statistical test: Two-tailed Mann–Whitney U-test (****p < 0.0001; *p < 0.05). Source data are provided as a Source data file.
