Fig. 3: Dosmit protein expression levels increase in wild-type flies throughout natural aging. | Nature Communications

Fig. 3: Dosmit protein expression levels increase in wild-type flies throughout natural aging.

From: Vesicular transport mediates the uptake of cytoplasmic proteins into mitochondria in Drosophila melanogaster

Fig. 3

a Western blot of Dosmit in flight-muscle tissue homogenates from wild-type flies aged 3 days, 1 week, 2 weeks, 4 weeks, 6 weeks, or 8 weeks. Dosmit* represents dimerized Dosmit. GAPDH: loading control. b Dosmit* expression levels based on the western blot of each age group. N = 2. Statistical test: two-tailed student’s t-test (**p < 0.01; *p < 0.05; ns: p = 0.0604). c Immunofluorescent staining showing mitochondria of wild-type flies aged 3 days, 2 weeks, 4 weeks, or 8 weeks. Scale bar: 10 μm. d Relative abundance of mitochondria of different sizes for each wild-type fly age group. N = 133, 144, 86, and 106 from left to right bars. Statistical test: Chi-Square test (****p < 0.0001). e Immunofluorescent staining showing mitochondria of Dosmit-EP flies aged 3 days, 2 weeks, 4 weeks, or 8 weeks. Scale bar: 10 μm. f Relative abundance of mitochondria of different sizes for each Dosmit-EP fly age group. N = 233, 211, 192, and 210 from left to right bars. Statistical test: Chi-Square test (ns: p = 0.0509, 0.1873, 0.8805 from left to right comparison). Source data are provided as a Source Data file.

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