Fig. 4: Dosmit-induced mitochondrial enlargement and formation of intramitochondrial vesicles in larvae and adult flies. | Nature Communications

Fig. 4: Dosmit-induced mitochondrial enlargement and formation of intramitochondrial vesicles in larvae and adult flies.

From: Vesicular transport mediates the uptake of cytoplasmic proteins into mitochondria in Drosophila melanogaster

Fig. 4

a Immunofluorescence and TEM images indicating that ectopic expression of Dosmit causes mitochondrial enlargement and the formation of double-membraned intramitochondrial vesicles in the muscles of both larvae and adult flies. Scale bar: 10 μm. b Mitochondrial sizes in Mef2 > Dosmit larvae (left) and adult flies (right) compared to Mef2/+ controls (mean ± SD). From left to right bars, N = 20 and 20 (larvae); 85 and 42 (adults). Statistical test: Two-tailed Mann–Whitney U-test (****p < 0.0001). c, d TEM images showing the double membrane (arrowheads) of the intramitochondrial vesicles in the flight muscles of Mef2 > Dosmit larvae (c) and adult flies (d). Scale bars for larvae images (left to right): 0.5 μm, 0.5 μm, and 100 nm. Scale bars for adult fly images: 1 μm (left) and 500 nm (right). Source data are provided as a Source Data file.

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