Fig. 7: IEGs associate with the NPC in NUP153-dependent manner during paused state and transcription initiation. | Nature Communications

Fig. 7: IEGs associate with the NPC in NUP153-dependent manner during paused state and transcription initiation.

From: Nucleoporin 153 links nuclear pore complex to chromatin architecture by mediating CTCF and cohesin binding

Fig. 7

a Immunostaining of LAMIN B1 and c-FOS DNA FISH in control and NUP153 KD HeLa cells are shown at the indicated time points. Cell numbers are as indicated. HeLa cells contain three c-FOS alleles (white arrows). Scale bar, 5 μm. b Cumulative frequency graphs showing distribution of the c-FOS locus distance to nuclear periphery in control and NUP153 KD HeLa cells at the indicated time points. Cumulative frequencies at a normalized distance (ND) of 0.0–0.12 are shown. See Supplementary Fig. 7 for the distribution of loci in all cells that were analyzed (ND of 0.0–0.5). ND = c-FOS locus to periphery distance/cell diameter (d), where d = (2× nuclear area/π)0.5. *p < 0.05; ***p < 0.001; Kolmogorov–Smirnov (KS)-test, n = 2 independent experiments. c Working model showing NUP153-mediated chromatin structure and transcription regulation at the IEG locus. Source data are provided as a Source Data file.

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