Fig. 2: XBD173 inhibits laser-induced vascular leakage and pathological choroidal neovascularization (CNV) in mice.

a Representative late phase fundus fluorescein angiography (FFA) images at indicated time points post laser injury. Scale bar: 200 µm. b Quantification of vascular leakage intensity after laser-induced CNV. 3 d n = 56; 7 d, 14 d n = 25 eyes from individual mice. c Quantification of vascular leakage area after laser-induced CNV. 3 d n = 56; 7 d, 14 d n = 25 eyes from individual mice. d Representative laser-induced CNV stained with isolectin B4 in RPE/choroidal flat mounts. Scale bar: 100 µm; FA fluorescein angiography. e Quantification of laser-induced CNV area in RPE/choroidal flat mounts. DMSO/XBD173 3 d n = 18/34; 7 d n = 38/17; 14 d n = 17/23 RPE/choroids from individual mice. f Pro-angiogenic growth factor levels in retinas of naïve and lasered mice at indicated time points. n = 8 eyes from individual mice. g Pro-angiogenic growth factor levels in RPE/choroids of naïve and lasered mice at indicated time points. n = 8 eyes from individual mice. h Representative infrared (IR) fundus images at indicated time points post laser injury. Lower panel shows OCT scan from one laser spot marked by a red line. Scale bar: 200 µm. I Quantification of laser spot size. DMSO/XBD173 0 d n = 106/71; 3 d n = 72/42; 7 d, 14 d n = 26 eyes from individual mice. Data show mean ± SEM and a linear mixed model was used for statistical analyses; *P < 0.05; **P < 0.01; and ***P ≤ 0.001. n.s., not significant. Source data are provided as a Source Data file.