Fig. 3: TSPO deficiency dampens phagocyte reactivity in laser-CNV.
From: The TSPO-NOX1 axis controls phagocyte-triggered pathological angiogenesis in the eye
a Representative images of Iba1+ cells within retinal laser lesions. Scale bar: 50 μm. b Quantification of Iba1+ cell morphology within lesions. n = 18 spots; TSPOΔMG 7 d n = 21 spots. c Quantification of Iba1+ area in lesions. n = 18 spots; TSPOΔMG 7 d n = 21 spots. d TSPO protein in retinas of naïve and lasered TSPOfl/fl and TSPOΔMG mice at indicated time points. Each lane represents an individual retina. Dotted line indicates individual blots processed in parallel. e Densitometry of western blots. LMW TSPO signals were normalized to β-Actin and HMW:LMW TSPO ratio determined. n = 6 retinas from two independent experiments. f Cytokines in retinas of naïve and lasered TSPOfl/fl and TSPOΔMG mice at indicated time points. CCL2 (n = 8 retinas from individual mice); IL-6, IL-1β, and TNF (naïve n = 13 retinas from individual mice); IL-6, TNF (3 d–14 d n = 8 retinas from individual mice) and IL-1β (6 h–14 d n = 10 retinas from individual mice). g Representative images of Iba1+ cells in RPE/choroidal lesions. Scale bar: 50 μm. h Quantification of Iba1+ cell morphology within lesions. n = 18 spots. i Quantification of Iba1+ area of the laser lesions. n = 18 spots. j TSPO protein in RPE/choroids of naïve and lasered TSPOfl/fl and TSPOΔMG mice at indicated time points. k Densitometry of Western blots. n = 6 RPE/choroids from two independent experiments. LMW lower molecular weight; HMW higher molecular weight. l Cytokines in RPE/choroids of naïve and lasered TSPOfl/fl and TSPOΔMG mice. CCL2 (n = 8 RPE/choroids from individual mice); IL-6 TSPOfl/fl/TSPOΔMG 6 h n = 11/12, 3–14 d n = 8; IL-1β TSPOfl/fl/TSPOΔMG 6 h n = 10/12, 3–14 d n = 10; TNF TSPOfl/fl/TSPOΔMG 6 h n = 9/12, 3 d n = 12 and 7–14 d n = 8 RPE/choroids from individual mice. m Extracellular ROS production by microglia from TSPOfl/fl and TSPOΔMG mice. Kinetics and area under the curve (AUC) are shown. n = 10 independent experiments (+XBD173, n = 3 independent experiments). Data show mean ± SEM. ROS data were analyzed using two-tailed unpaired Student’s t test. A linear mixed model was used for laser-CNV data; **P < 0.01 and ***P ≤ 0.001. Source data are provided as a Source Data file.
