Fig. 3: Pi accumulation by Synechococcus sp. WH8102.
Left (a, b) and right (c, d) panels show the results of two representative pulse-chase experiments with cells at exponential and early stationary growth phases, respectively. a, c Accumulation dynamics of 10−8 mol l−1 33Pi-pulse added at 0 h in live cells washed with artificial seawater − the total Pi (black circles), live cells washed with deionized water − the cytoplasmic Pi (white circles) and in the PFA-fixed cellular biomass − the macromolecule-bound Pi (red circles), as a percentage of added tracer. b, d Accumulation dynamics of the 10−6 mol l−1 32Pi-chase added at 2 h as the total Pi, the cytoplasmic Pi and the macromolecule-bound Pi. The additional right Y-axis (b, d) shows cellular rates of 32Pi-chase accumulation. To assist comparison of the labile and non-labile Pi, the areas below lines are coloured yellow for the labile Pi and red for non-labile Pi. Symbols are connected with lines to indicate the slopes of the 33Pi and 32Pi tracer accumulation. The X-axis is broken to add an additional, final time point. Circles present mean values of technical duplicates or individual measurements. Source data are provided as a Source Data file.
