Fig. 5: Fungal colonization alters early-life systemic immunity in mice.
From: Intestinal fungi are causally implicated in microbiome assembly and immune development in mice
Percentage of a unstimulated splenic cell populations, and b cytokine-producing unstimulated splenocytes from 4-week-old gnotobiotic mice. Cells were stained with intra- and extracellular marker-specific antibodies and quantified by flow cytometry. c Serum antibody concentrations detected by electrochemiluminescence (MSD). a–c Data expressed as mean ± S.E.M. Color denotes colonization treatment (GF = yellow, B = red, Y = green, BY = royal blue, BY + Abx = cyan blue, BY + Afx = purple); NGF = 5, NB = 6, NY = 8, NBY = 6, NAbx = 5, NAfx = 6; different letters above bars indicate statistically significant differences defined by one-way ANOVA and Tukey post hoc tests; P < 0.05. d Heat map of biweight correlations between merged 16S (black) and ITS (blue) ASVs of the same species (x-axis) and reported immune features (y-axis) at week 4. Color denotes positive (red) and negative (blue) correlation values. Significant correlations are denoted with a cross and defined by the BiCOR method with FDR correction; P < 0.05. Source data are provided as a Source data file.
