Fig. 4: Dmc1−/− Puma−/− Noxa−/− females generate embryos. | Nature Communications

Fig. 4: Dmc1−/− Puma−/− Noxa−/− females generate embryos.

From: The BCL-2 pathway preserves mammalian genome integrity by eliminating recombination-defective oocytes

Fig. 4

a Heatmap showing chromosome gains and losses in Dmc1−/− Puma−/− Noxa−/− zygotes and their matched polar body, showing multiple reciprocal chromosomal aneuploidies. b Development of embryos from control (n = 3 females, n = 48 zygotes, N = 2 experimental repetitions) and Dmc1−/− Puma−/− Noxa−/− females (n = 3 females, n = 37 zygotes, N = 2 experimental repetitions). Dotted circles outline paternal and maternal pro-nuclei. Asterisks indicate second polar body. Time shows hours from zygote collection. Scale bar 100 µM. c t-SNE plot of single-cell RNA-seq analysis of control and Dmc1−/− Puma−/− Noxa−/− blastomeres. Dotted circles represent pairs of blastomeres derived from the same embryo. Control (Ctrl) is Dmc1+/− Puma+/− Noxa+/−.

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