Fig. 3: Identification of alternative splicing (AS) events and differential splicing events in seven stages of preimplantation mouse embryos.

a Schematic diagram of the seven types of AS events. b Distributions of AS events in the seven stages. The percentages (bar) and total numbers (red line and indicated numbers) of AS events in the seven classes are presented in each stage. c Distribution of AS genes in the seven stages. The percentages (bar) and total numbers (red line and indicated numbers) of AS genes in the seven classes are presented in each stage. The percentages at the top of the bars represent the proportions of total AS genes among total genes with multiple isoforms in each stage. d Numbers of long-read transcripts gained or lost in consecutive stages. The percentages at the top of the bars represent the proportions of gained transcripts among the total transcripts of the later stage, and the percentages at the bottom of the bars represent the proportions of lost transcripts among the total transcripts of the previous stage. e–k Numbers of AS events of seven types, which were gained or lost in consecutive stages. The percentages in the top of bars represent the proportion of gained events relative to the total AS events in the latter stage, and the percentages in the bottom of bars represent the proportion of lost events relative to the total AS events in the previous stage. e for SE, f for A3, g for A5, h for AF, i for RI, j for MX and k for AL.