Fig. 1: Blue-light-induced activity of PqsL.
From: Photoinduced monooxygenation involving NAD(P)H-FAD sequential single-electron transfer
a Reaction of PqsL within the alkyl quinolone biosynthetic pathway. The downstream/parallel reaction of PqsBC was used throughout the study for coupled assays to quantify PqsL catalytic activity. 2-HABA is unstable with a t½ of 16 minutes. 2-ABA—2- aminobenzoylacetate; HHQ—2-heptyl-4(1 H)-quinolone; 2-HABA—2-hydroxylaminobenzoylacetate; HQNO—2-heptyl-4-hydroxyquinoline-N-oxide; oCoA—octanoyl-CoA. b HQNO formation in the coupled enzyme assay as a function of light intensity (LED source, ʎmax 466 nm, 20 nm full width at half maximum, n ≥ 6). c HQNO formation in the coupled enzyme assay depending on the PqsL electron donor (n ≥ 4). The flavin reductase HpaC was used to generate reduced flavin in situ for PqsL light-independent activity with NADH. Error bars denote SD of independent experiments. MNAH—1-methyl-1,4-dihydronicotinamide, TEA—triethylamine.
