Fig. 4: Spectroscopic assessment of flavin conformation and FAD-binding equilibria in PqsL.
From: Photoinduced monooxygenation involving NAD(P)H-FAD sequential single-electron transfer
a Circular dichroism spectra of FAD in solution (orange), PqsL (black), PqsL+2-ABA (blue) and PqsL-Q (red) (one representative measurement). b Dilution series of PqsL, monitored by FAD fluorescence polarization, to deduce KD of PqsL- (black), PqsL-2ABA- (blue), and PqsL-Q- (red) complexes with FAD (error bars indicate SD from three independent experiments). KD values were 249.5 ± 29.8 nM for the PqsL-, 259.8 ± 19.2 nM for PqsL-2ABA- and 37.5 ± 6.7 nM for the PqsL-Q-FAD complex.
