Fig. 2: Phenotypic and transcriptomic analyses during regeneration.
a A cartoon of time-dependent amputation and regeneration transcriptome sequencing in earthworm. b Snapshots of cross sections at six time points after post-amputation. c HE staining of cross sections at six time points post-amputation. The different structure layers were labeled. ED: epidermis, CM: circular muscle, LM: longitudinal muscle, IN: intestine. A representative scale bar (100 μm) was showed. The HE staining experiments were independently repeated (three times) and obtained similar results. d Detection of cell proliferation at different time points post-amputation using a marker Ki-67 by Immunofluorescent double staining. The red fluorescence represented signals and the blue fluorescence represented cell nucleus. Similar results were reproduced in two independentbiological experiments. e, Number comparisons of DEGs at different regeneration time points, compared to regeneration 0 h (controls). Time points included 0, 6, 12, 24, 48 and 72 h. f Co-activition of gene expression in early regeneration processes between earthworm and planarian. The early growth response genes and transcriptional factor genes respectively were compared for two species (earthworm and planarian). The planarian gene 1080 expression changes were obtained from a previous study37. The dark red represented genes with higher expression levels, and the dark blue showed genes with lower expression levels.
